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Updated: May 2, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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小型分子有機プローブを用いた3Dサブ10 nmマルチカラー展開STEDナノ顕微鏡検査プロトコル

Jing Yan1, Yubing Han1, Xiang Hao1

  • 1State Key Laboratory of Extreme Photonics and Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, China.

STAR protocols
|December 12, 2025
PubMed
まとめ

本研究では、小型分子有機プローブ（SMOP）と適応光学（AO）を、展開刺激放出枯渇（ExSTED）顕微鏡検査と組み合わせた新しい手法を紹介する。この高度な技術により、深い生物学的イメージングでサブ10 nmの解像度が得られる。

キーワード:
細胞生物学細胞培養顕微鏡検査

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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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