DNA指向型エレクトロキシルミネッセンスナノスフィアと電気触媒強化マイクロ流体アレイによる迅速な多菌検出

Rapid detection of multibacterial pathogens is crucial for accelerating the diagnosis and treatment of bacterial infections. We propose a rapid and efficient electrochemiluminescence (ECL) sensor for the synchronous detection of multiple bacterial pathogens, including Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and methicillin-resistant S. aureus. This homogeneous sensor is based on a self-assembled DNA nanosphere loaded with tetrakis (4-carboxyphenyl) porphyrin (TCPP). The sensor operates in an "off-on" mode, in which bacterium-aptamer binding triggers a conformational change in the DNA nanosphere, releasing TCPP and generating an enhanced ECL signal. The inclusion of cerium nanoparticles boosts signal intensity through electrocatalytic reactions, improving sensitivity with a detection limit of ≤100 colony-forming units per milliliter. Integrated with a microfluidic chip, the system enables multibacterial detection in just 45 minutes. Bacterial quantification in clinical samples strongly correlates with digital polymerase chain reaction results. This approach provides a rapid, specific, and efficient diagnostic tool for bacterial infections with great potential for point-of-care applications in clinical settings.