トリプルネガティブ乳がんにおけるイメージングおよび細胞相互作用のためのカーボンナノドットナノ粒子の探索
Mehrnoosh Bahadorani1, Kerui Wu2, Jianjun Wei2
1Department of Nanoengineering, Joint School of Nanoscience & Nanoengineering, North Carolina Agriculture and Technical State University, Greensboro, NC, USA.
International journal of nanomedicine
|December 15, 2025
まとめ
カーボンナノドット（CND）は細胞イメージングに有望ですが、トリプルネガティブ乳がん細胞でアポトーシスを誘発します。これらのナノマテリアルの生体適合性を確認するには、標準的な生存率試験では不十分です。
科学分野:
- Nanotechnology
- Biomedical Engineering
- Cell Biology
背景:
- Carbon dot nanoparticles (CNDs) are recognized for their fluorescence and synthesis ease, making them suitable for cellular imaging.
- However, a comprehensive understanding of their biological impact is lacking, necessitating further investigation.
研究 の 目的:
- To synthesize E-CNDs (ethylenediamine and citric acid-based carbon nanodots) via microwave-assisted methods.
- To investigate the intracellular distribution and biological effects of E-CNDs on triple-negative breast cancer (TNBC) cells.
主な方法:
- E-CNDs were synthesized and incubated with TNBC cells.
- Intracellular localization was assessed using imaging.
- Cytotoxicity was evaluated using CCK-8 assays.
- Apoptotic activity was measured via Annexin V/PI staining and molecular markers (G6PDH, procaspase 3).
主要な成果:
- E-CNDs exhibited perinuclear localization and mitochondrial presence, suitable for intracellular imaging.
- Standard CCK-8 assays indicated no significant cytotoxicity.
- Molecular analysis revealed dose-dependent downregulation of G6PDH and upregulation of procaspase 3.
- Annexin V/PI staining confirmed increased apoptotic activity.
結論:
- E-CNDs function as effective imaging agents with no apparent cytotoxicity via standard assays.
- However, E-CNDs induce molecular and apoptotic responses, indicating potential biological effects.
- Cell viability assays alone are insufficient for assessing nanomaterial biocompatibility; detailed molecular and functional evaluations are crucial for safe biomedical applications.
