糖尿病創傷治癒のためのアロエ抽出物負荷カチオン化シルクフィブロイン/酸化ヒアルロン酸注入可能自己修復ヒドロゲル

Chronic wound healing in diabetes mellitus is a major clinical challenge, and its pathological microenvironment presents a vicious cycle of high glucose, hypoxia, chronic inflammation, and cellular dysfunction, which makes it difficult for a single traditional dressing to effectively promote re-epithelialization, collagen deposition, and vascular regeneration. We propose a novel injectable self-healing dual-crosslinked hydrogel integrating both "dynamic crosslinking" and "covalent binding" designed to possess multifunctionality including antibacterial, antioxidant, anti-inflammatory, and pro-healing properties. The hydrogel is formed via Schiff base reaction between aminated cationic silk fibroin (CSF) and oxidized sodium hyaluronate (OSH), further stabilized using an EDC/NHS activation system. This dual-network hydrogel platform is injectable and capable of forming in situ at specific sites, and has robust self-healing capability. Its porous structure, loaded with bioactive aloe extract (AE), imparts antibacterial, antioxidant, and anti-inflammatory properties, and significantly enhances cell adhesion and migration efficiency. Animal studies further demonstrated that this hydrogel effectively accelerates the healing of diabetic wounds by promoting re-epithelialization, accelerating angiogenesis and collagen remodeling evidenced by the upregulation of CD31 and α-SMA markers, and modulating the inflammatory response via downregulation of the pro-inflammatory cytokine IL-1β.