関連する実験動画

Updated: Jun 22, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

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RT-LAMPとCRISPR-Cas12bベースの戦略を組み合わせたHCV RNA検出のための迅速アッセイ

Avisha Chowdhury1, Bianca Garcia Garcia2, Muhammad Atif Zahoor1

  • 1Toronto Centre for Liver Disease, Toronto General Hospital, University Health Network, University of Toronto, Canada.

The Journal of infectious diseases
|December 15, 2025
PubMed
まとめ

新しい迅速で費用対効果の高いC型肝炎ウイルス（HCV）RNA検査、RT-LAMPとCRISPR-Cas12bを使用し、スクリーニングを強化。この高感度アッセイは45分以内に血漿中のHCV RNAを検出し、遠隔地の集団の診断を改善する。

キーワード:
CRISPR-Cas12bHCVアッセイHCV診断C型肝炎ウイルスPOCTポイントオブケア検査RT-LAMP

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関連する概念動画

CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...