SUPER：精密遺伝子発現制御、リーケージ最小化、遺伝子回路安定化のための遺伝子部品のアップサイクリング

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Updated: Jan 8, 2026

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

Taeyang Heo¹, Dongwon Park¹, Woosub Shin¹

¹Department of Life Sciences, Pohang University of Science and Technology, Pohang, 37673, South Korea.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|December 15, 2025

Abstract:

A long-standing goal of synthetic biology is to reprogram cells by rewiring genetic parts. Despite the expanding library of genetic parts, construction of integrated synthetic circuits with desired specifications remains challenging in part due to intricate dependence on sequence contexts, where unexpected narrow dynamic ranges and leaky expression can plague system performance. To provide an alternative approach to the screening process of iterative design-build-test cycles, SUPER (Synthetic Upcycling Platform for Engineering Regulators), a modular platform for upcycling genetic devices is introduced. Inspired by antagonistic regulation mechanisms, SUPER employs small RNA as an add-on controller to modulate gene expression patterns without genetic modification of target regulators. SUPER not only enhances the performance of RNA-, chemical-, temperature-, and protein-responsive regulators up to 1011%, but also allows to cover an expanded dynamic range up to 22 018.9-fold. This enhanced control can provide genetic circuit stability, particularly under strong selective pressures, as demonstrated with a Holin-expressing kill switch integrated with SUPER, maintaining stable functionality for over 30 days. Finally, SUPER combines with an environmental sensor, TlpA36, functioning as a chemical- and temperature-responsive 2-input kill switch. Featuring straightforward design, minimal cellular burden, and expanded tunability, SUPER provides a systematic upcycling framework for genetic circuit construction in biotechnology.

キーワード:

バイオセンサー遺伝子回路の安定性遺伝子デバイスキルスイッチリーケージ最小化小RNA 合成生物学

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Rapid Characterization of Genetic Parts with Cell-Free Systems

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Published on: August 30, 2021

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