レフスモリス由来の4NsL染色体断片はコムギに縞枯病およびフザリウム頭枯病抵抗性を付与する

Leymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) serves as a tertiary genetic pool for wheat improvement, harboring valuable traits such as disease resistance and stress tolerance. To harness this potential, we characterized two novel wheat-L. mollis translocation lines, M892 and M956, derived from a cross between common wheat and a 4Ns disomic addition line. Phenotypic evaluation revealed that M956 displays high resistance to both stripe rust and Fusarium head blight, while M892 is susceptible; both lines exhibit enhanced grain weight, with M892 additionally showing a dwarf phenotype. Cytogenetic analyses identified M892 as a T5DL-4NsL-5DL·5DS insertional translocation line and M956 as a T4NsL-5DL·5DS terminal translocation line. Genetic mapping localized the stripe rust resistance to a critical 98 Mb region on chromosome 4NsL, and comparative transcriptomics pinpointed 47 candidate resistance genes uniquely upregulated in M956. Furthermore, we developed eight 4NsL-specific molecular markers, providing a practical tool for the marker-assisted utilization of these resistance genes in wheat breeding programs.