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CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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関連する実験動画

Updated: Jun 30, 2026

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
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Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

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ゼブラフィッシュにおける最適化CRISPR干渉を用いた系統的エンハンサーマッピングと機能解析

Jiulin Chan1,2, Zhichao Wu1,2, Mingli Liu1,2

  • 1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.

Nucleic acids research
|December 19, 2025
PubMed
まとめ
この要約は機械生成です。

研究者らは、遺伝子調節を研究するためにゼブラフィッシュにおけるクラスター化常在性短回文繰り返し配列干渉（CRISPRi）を最適化しました。このシステムは、ヒレと血球細胞の発生を制御する新規エンハンサーを特定し、シス調節要素アノテーションを進歩させました。

キーワード:
CRISPRiゼブラフィッシュエンハンサー遺伝子調節ゲノミクス発生生物学

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関連する実験動画

Last Updated: Jun 30, 2026

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科学分野:

  • ゲノミクス
  • 発生生物学
  • 分子遺伝学

背景:

  • 非コードシス調節要素、特にエンハンサーは、遺伝子発現制御に不可欠です。
  • ゼブラフィッシュモデルにおけるクラスター化常在性短回文繰り返し配列（CRISPR）干渉（CRISPRi）を用いたインビボでのエンハンサー機能の研究は困難でした。

研究 の 目的:

  • ゼブラフィッシュにおけるCRISPRiシステムの効率的な遺伝子抑制のための最適化。
  • 最適化されたCRISPRiシステムを用いたグロビン遺伝子の遠位エンハンサーの機能的アノテーション。
  • 脊椎動物におけるシス調節要素アノテーションのための統合プラットフォームの開発。

主な方法:

  • 効率的な遺伝子ノックダウンのために構成要素濃度を微調整することにより、CRISPRiシステムを最適化しました。
  • グロビン遺伝子の遠位エンハンサーの機能的特性評価にCRISPRiを適用しました。
  • ゲノムワイドなエンハンサー-プロモーター（EP）相互作用のマッピングにHi-Cおよびヒストン修飾アッセイを利用しました。

主要な成果:

  • ヒレと血球細胞の発生に影響を与える、これまで報告されていなかった有意な調節強度を持つエンハンサーを特定しました。
  • 434のエンハンサー-プロモーター相互作用をマッピングし、新規調節ループを明らかにしました。
  • 統合アプローチの有効性を示す、いくつかの新規EPループを検証しました。

結論:

  • 最適化されたCRISPRiシステムは、ゼブラフィッシュにおけるエンハンサーの機能的研究を強化します。
  • 本研究は、シス調節要素アノテーションのための計算および実験的方法を統合する堅牢なプラットフォームを提供します。
  • 本研究は、脊椎動物の発生および疾患における遺伝子調節の理解を進歩させます。