ポテトの成長、収量、栄養含有量の改善のための液体リン酸溶解性細菌バイオ肥料の製剤と圃場性能

In the present study, a liquid formulation(s) containing two phosphate-solubilizing Bacillus licheniformis PRPSB₁₀ and Pseudomonas putida PRPSB₃₈ has been developed and tested for its efficacy to improve growth, yield, and nutrient content of potato (Solanum tuberosum L.) under integrated nutrient management system. These two phosphate-solubilizing strains, isolated from potato rhizosphere showed high phosphate-solubilizing efficiency, with solubilization index of 6.1 and 5.8, respectively, on modified Pikovskaya agar. The strain PRPSB₁₀ solubilized 659.9, 202.0, and 113.7 μg ml^-1 of tricalcium phosphate, rock phosphate, and iron phosphate, respectively, while the strain PRPSB₃₈ solubilized 457.4, 187.9, and 80.0 μg ml ^-1 of the same substrates, respectively, after 5 days of incubation in NBRIP broth. Among the different cell protectants tested, 0.1% carboxymethyl cellulose (CMC) was found to be the best for B. licheniformis PRPSB₁₀ while 5 mM trehalose was best suited for P. putida PRPSB₃₈ maintaining the cell viability ~10⁸ CFU ml^-1 up to 1 year under ambient storage conditions. Two-year field evaluations of the liquid formulation in potato variety Kufri Pukhraj with different P dosages revealed a significant improvement in plant growth, phosphorus content, tuber yield and soil fertility over the respective control treatment with an average increase of 16.2% in yield over control. Economic analysis further indicated mean net returns of ₹44,876 ha^-1 and an improved benefit-cost ratio of 2.52 compared with the uninoculated control. The results indicated that the developed liquid PSB formulation exhibited excellent stability, effectively enhanced yield and phosphorus availability highlighting its potential to be used as a liquid biofertilizer for improving potato productivity and maintaining soil health.