アポE COG 133 ミメティックペプチドは、マウスにおける生存率、感染量、およびクロストリジオイデス・ディフィシル毒素A誘発性腸管障害を改善する
Orleâncio G R de Azevedo1, Jae H Shin2, Rosemayre S Freire3
1Laboratory of the Biology of Tissue Healing, Ontogeny and Nutrition, Department of Morphology and Institute of Biomedicine, School of Medicine, Federal University of Ceara, Fortaleza, Brazil.
Abstract:
Apolipoprotein E (ApoE = protein; APOE = gene), a lipid carrier that modulates inflammatory responses, may influence Clostridioides difficile (C. difficile) infection (CDI) outcomes. We explored the role of the APOE gene using apoE-deficient mice challenged by C. difficile toxin A (TcdA)-induced enteritis, and the potential use of the ApoE mimetic peptide in repairing the intestinal damage induced by TcdA. 4-cm ileal loops from C57BL/6 wild-type and APOE knockout (-/-) were ligated and injected with either PBS or TcdA (50 µg). After 4 h of incubation, the intestinal loops were harvested for measurement of length, weight, volume of secretion, and histopathology scores. In mouse ileal loops, TcdA induced a significant increase in weight/ileal loop length in the wild-type mice. When APOE-/- mice were infected with 1 × 104-105 CFUs of C. difficile, they had higher deaths and diarrhea scores compared to wild-type. APOE-/- mice under the toxin A (TcdA) had worse inflammatory changes in the ileal loop. APOE-/- mice treated with COG133 (3 mg/kg) showed fewer deaths, and lower diarrhea scores, but no change in C. difficile shedding. This suggests a potential anti-inflammatory role of COG133 in CDI. More studies are neede to these intial findings in depth.
