多層構造と成長因子を有する多機能フィルムによる上皮組織および骨組織の同時再生

Although guided bone regeneration (GBR) membranes are frequently utilized in oral-maxillofacial surgery, there continues to be a demand for membranes that can concurrently facilitate epithelial sealing and bone regeneration. In this work, a multilayered polycaprolactone (PCL) film, incorporating a central dense barrier layer and leaf-stacked structure layers on both surfaces for cell/tissue adhesion and bioactive molecule loading (MFLSS), was produced via a heating-cooling method using tetraglycol. Platelet-derived growth factor-BB (PDGF-BB) and bone morphogenetic protein-2 (BMP-2) were incorporated into the porous leaf-stacked layers on each side to promote epithelial and bone tissue regeneration, respectively. The PDGF-BB and BMP-2 embedded in the leaf-stacked layers were released in a sustained manner at therapeutic concentrations for 15 and 17 days, respectively. In vitro and in vivo assays indicated that the PDGF-BB-loaded layer significantly improves cell/tissue adhesion as well as cell migration, while the BMP-2-immobilized layer effectively induces osteogenic differentiation and bone formation. Collectively, these findings indicate that the multifunctional film serves as a promising GBR membrane by consistently sealing the defect site and accelerating bone healing.