ヒト羊膜由来間葉系幹細胞の分離および免疫調節能評価プロトコル

Mesenchymal stem cells (MSCs) are promising for regenerative medicine and cell-based therapies due to their multipotent and immunomodulatory properties. Here, we present a protocol for isolating MSCs from the amnion of the human amniotic membrane (AM-MSCs) using the explant culture approach and their subsequent characterization. We also describe steps for evaluating the immunomodulatory potential of the conditioned medium (CM) harvested from AM-MSCs, enabling assessment of its secretome without direct cell-to-cell contact.