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Updated: Jan 8, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

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超高感度空間組織プロテオミクスワークフロー：1日あたり100以上のプロテオームを実現

Melissa Klingeberg1, Christoph Krisp2, Sonja Fritzsche1

  • 1Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Spatial Proteomics Group, Berlin, Germany; MDC-Bruker Center of Excellence for Single Cell Omics, Berlin, Germany; Humboldt University of Berlin, Faculty of Life Science, Berlin, Germany.

Molecular & cellular proteomics : MCP
|December 19, 2025
PubMed
まとめ

最適化された空間組織プロテオミクスワークフローは、高解像度のプロテオーム解析を可能にします。本研究では、7,500以上のタンパク質定量化を達成し、大規模な翻訳研究をサポートするcellenONEプロトコルとEvosep ONE Whisper Zoomグラジエントについて詳述しています。

キーワード:
空間組織プロテオミクスプロテオーム解析Evosep ONEDIA MS翻訳研究

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Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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