Dentin Remineralization Associating Peptide- and Particle-Assisted Strategies

科学分野:

• Biomaterials Science
• Dental Research
• Mineralization Studies

背景:

• Dentin demineralization poses a significant challenge in restorative dentistry.
• Current remineralization strategies often have limitations in efficacy and speed.
• Investigating novel combinations of biomaterials can lead to improved treatment outcomes.

研究 の 目的:

• To evaluate the efficacy of the polymer-induced liquid precursor (PILP) process for dentin remineralization.
• To assess an experimental composite containing dicalcium phosphate dihydrate (DCPD) particles for dentin remineralization.
• To determine if combining PILP pretreatment with DCPD composite enhances dentin remineralization compared to individual methods.

主な方法:

• Artificial dentin lesions were created and divided into four groups: control, PILP, DCPD, and PILP + DCPD.
• Remineralization was assessed over 28 days using mineral-to-matrix ratio (MMR) via FTIR and nanoindentation for elastic modulus (EM).
• Microscopy techniques (SEM, EDS, TEM, SAED) were used for morphological and compositional analysis.

主要な成果:

• All groups showed MMR recovery, but only the PILP + DCPD group reached sound dentin levels by 14 days.
• The PILP + DCPD group exhibited significantly higher elastic modulus (EM) in both external and internal lesion areas compared to control.
• EM recovery in the PILP + DCPD group reached 57% of sound dentin, significantly higher than PILP (18%) or DCPD (31%) alone.

結論:

• The combination of PILP pretreatment and DCPD composite significantly enhances dentin remineralization.
• This synergistic approach restores both mineral content and mechanical properties of demineralized dentin more effectively.
• The PILP + DCPD strategy represents a promising advancement for treating artificial dentin lesions.