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Updated: Jan 8, 2026

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Published on: December 9, 2022

患者由来iPSCsから視細胞様細胞への分化プロトコル

Yang Pan¹, Takeshi Iwata¹

¹Division of Molecular and Cellular Biology, National Institute of Sensory Organs, NHO Tokyo Medical Center, Tokyo 152-8902, Japan.

|December 20, 2025

Abstract:

Retinal biopsies are rarely feasible, limiting direct studies of inherited retinal dystrophies (IRDs). We present a protocol modeling occult macular dystrophy, an autosomal-dominant IRD, using induced pluripotent stem cells (iPSCs) generated from patient blood cells. We describe steps to differentiate iPSCs into photoreceptor-like cells by transducing CRX and NEUROD1 via a tetracycline-inducible PiggyBac vector. We also detail procedures for the validation of photoreceptor markers to assess differentiation. This protocol enables mutation-focused investigation of IRD pathogenesis. For complete details on the use and execution of this protocol, please refer to Pan et al.¹.

キーワード:

細胞分化遺伝学健康科学分子生物学幹細胞

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