複雑なin vitro腸管モデルにおける粘膜および血管バリアの独立した測定のための適合可能なデバイス

Electrochemical approaches for monitoring cell barrier integrity have become important read-out tools for in vitro models. Conventional commercially available electrochemical devices are however poorly suited for monitoring the more complex and multi-dimensional models which have recently been developed thanks to advances in tissue engineering. In particular, commercially available devices are typically unable to provide barrier-by-barrier resolution in multi-barrier models. In this study, a gut-immune-vasculature model is presented and utilized to investigate the potential for a novel conformable device to independently monitor two cell barriers within the same model-device platform. The platform, supported by cytokine analysis, is first used to study known bacterial metabolites and dietary compounds, showing changes in epithelial and/or endothelial barrier resistance. The platform is also used to test complex mixtures of bacterial metabolites produced by four intestinal bacteria individually as well as by a community of 25 diverse gut bacteria. The study demonstrates the potential for employing tailored in vitro intestinal models coupled with novel electrochemical device monitoring for host-microbe interactions studies.