ヒト血漿中の神経線維軽鎖に対する選択性のDNAアプタマーの競合的SELEX発見

Miyu Matsumoto¹, Kazunori Ikebukuro¹, Kaori Tsukakoshi²

¹Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan.

Biochemical and biophysical research communications

|December 21, 2025

Abstract:

Neurofilament light chain (NfL) is a blood-based biomarker closely associated with neurodegeneration and the progression of Alzheimer's disease. Here, we report the identification of novel single-stranded DNA aptamers that specifically recognise NfL with high affinity. Using a competitive SELEX strategy incorporating NfL, we enriched aptamer candidates that selectively bind the clinically relevant region of NfL. Two lead aptamers, MN711 and MN734, displayed nanomolar dissociation constants of 11 nM and 8.1 nM, respectively, comparable to those of widely used anti-NfL antibodies. Circular dichroism spectroscopy confirmed parallel G-quadruplex structures, and both aptamers showed minimal off-target binding to amyloid β40, amyloid β42, or phosphorylated tau181. Importantly, MN711 retained concentration-dependent binding to NfL spiked into human plasma. These aptamers provide promising ligand candidates for NfL biosensing platforms and blood-based diagnostic devices for neurodegenerative diseases.

キーワード:

血液ベースのバイオマーカー DNAアプタマー神経線維軽鎖

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