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関連する概念動画

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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What is Gene Expression?01:36

What is Gene Expression?

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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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What is Gene Expression?01:42

What is Gene Expression?

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Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
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Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

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Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
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General Transcription Factors01:30

General Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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関連する実験動画

Updated: Jan 8, 2026

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
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α-因子によって調節される遺伝子発現プログラムを特定した酵母のグローバルトランスクリプトーム解析

Soojin Yeom1,2,3,4, Jung-Shin Lee1,2

  • 1Department of Molecular Bioscience, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Republic of Korea.

Journal of microbiology and biotechnology
|December 21, 2025
PubMed
まとめ

この研究は、α-因子フェロモンに応答する酵母細胞におけるゲノムワイドな遺伝子発現変化を明らかにする。交配、細胞壁再構築、細胞周期調節に関与する数百の遺伝子を同定する。

キーワード:
Saccharomyces cerevisiae細胞周期停止クロマチンリモデリングフェロモンシグナル伝達α-因子

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Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow
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Temporal Quantification of MAPK Induced Expression in Single Yeast Cells
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科学分野:

  • 分子生物学
  • 酵母遺伝学
  • 細胞シグナル伝達

背景:

  • 酵母の交配フェロモンであるα因子は、接合型aの細胞にシグナル伝達カスケードを誘導する。
  • 既知の生理学的応答には、G1細胞周期停止、極性成長（シュムー形成）、および細胞融合が含まれる。
  • 包括的なゲノムワイドなトランスクリプトーム解析は、これまで欠如していた。

研究 の 目的:

  • α因子処理を行った接合型aの酵母細胞における転写変化のゲノムワイドな解析を実施する。
  • フェロモンシグナル伝達に応答する遺伝子発現変化（DEG）を同定する。
  • 酵母の交配応答を理解するためのリソースを提供する。

主な方法:

  • RNAシーケンシング（RNA-seq）を用いて遺伝子発現をプロファイリングした。
  • 酵母の接合型a細胞にα因子を処理した。
  • 遺伝子オントロジー（GO）解析を用いてDEGの機能的濃縮を解釈した。

主要な成果:

  • 957の差次的発現遺伝子（DEG）を同定した：448個がアップレギュレートされ、509個がダウンレギュレートされた。
  • アップレギュレートされた遺伝子は、フェロモンシグナル伝達、細胞壁生合成、および細胞形状調節に濃縮されていた。
  • ダウンレギュレートされた遺伝子は、細胞周期進行、クロマチンリモデリング、およびヌクレオソーム組織化に関連していた。

結論:

  • 本研究は、酵母のフェロモン応答性遺伝子の貴重なデータセットを提供する。
  • 交配応答に関与する新規候補遺伝子が同定された。
  • 本研究の結果は、酵母のフェロモンシグナル伝達における転写調節とクロマチンダイナミクスの協調に関する洞察を提供する。