多機能性硫化銅ナノ粒子堆積による象牙質接着の強化：相乗的な脱水、MMP阻害、および抗菌作用

To develop a multifunctional strategy based on in-situ copper sulfide (CuS) nanoparticle deposition, aiming to simultaneously mitigate interface-confined water to improve adhesive infiltration, suppress enzymatic degradation, and prevent bacterial colonization.

Demineralized dentin matrices (DDM) were sequentially treated with CuSO₄ and Na₂S solutions at three concentrations (0.0015, 0.015, 0.15 mol/L), with conventional wet-bonding as a control. Nanoparticle distribution, matrix dehydration, matrix metalloproteinase (MMP) activity, antibacterial efficacy (Streptococcus mutans, Staphylococcus aureus and Escherichia coli), and bonding performance (nanoleakage, microtensile bonding strength) were systematically evaluated.

Uniform CuS deposition significantly reduced DDM hydration to release the interface-confined water. The 0.015 and 0.15 groups showed enhanced mechanical properties. Moreover, all concentrations of CuS deposition inhibited MMP and showed antibacterial effect. As a result, the 0.015 and 0.15 groups showed improved adhesive infiltration, reduced nanoleakage (p < 0.05) and increased both immediate and aged microtensile bonding strength (p < 0.01).

In-situ CuS nanoparticle deposition synergistically enhances bond durability, through DDM dehydration, MMP inhibition, and antibacterial action. This approach effectively minimizes hybrid layer defects and collectively prolongs bonding longevity.

The Cu-assisted bonding technique provides a clinically feasible solution to address multifactorial failure modes in dentin bonding, leveraging nanomaterial synergy for durable adhesive restorations.