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Updated: Jan 8, 2026

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
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ΔBOP最適化によるハイスループット機能細胞選別における大粒子回収率の向上

Naohisa Sakamoto1, Eikichi Shibata1,2, Mitsuo Yoshimura3

  • 1Life Science Technology Research & Development Department, Technology Development Laboratories, Sony Corporation, Tokyo, Japan.

Scientific reports
|December 21, 2025
PubMed
まとめ

フローサイトメトリーにおける大粒子の選別は困難です。この研究では、液滴の安定性を向上させ、単一細胞解析のための回収率を大幅に向上させる新しいΔBOP戦略を紹介します。

キーワード:
フローサイトメトリー機能的細胞解析大粒子選別回収率ΔBOP（デルタブレークオフポイント）

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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