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Updated: Jan 8, 2026

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm

Published on: January 28, 2011

マメ科作物のDNAメチル化：メカニズム、農学的関連性、および展望

An Xie¹, Kang Ning^1,2, Weixin Zhou¹

¹International Joint Laboratory for Agricultural Plant Metrology and Equipment Innovation, College of Life Sciences, China Jiliang University, Hangzhou, 310018 China.

Molecular breeding : new strategies in plant improvement

|December 22, 2025

Abstract:

As the second most cultivated crop group, legumes occupy vast cultivated areas and hold immense agricultural economic value. DNA methylation, a highly conserved epigenetic modification in legumes, is dynamically regulated by various methylation enzymes. In recent years, investigating the functional roles of DNA methylation in legumes has emerged as a pivotal research area, yet comprehensive reviews in this area remain limited. This review discusses de novo DNA methylation in plants, along with the dynamic mechanisms governing its maintenance, recognition, and removal, and further explores the applications of DNA methylation in legume domestication and improvement; its role in responses to biotic and abiotic stresses, and its impact on genomic stability and transcriptional regulation. Additionally, this review highlights the potential of DNA methylation in advancing legume breeding. By synthesizing current knowledge, this review provides valuable theoretical insights into the application of DNA methylation for legume improvement and genome optimization. It also offers valuable guidance for leveraging epigenetic modifications in legume breeding, improving nitrogen fixation efficiency, and regulating the expression of vital plant proteins.

キーワード:

農学的関連性 DNAメチル化マメ科メカニズム

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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

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Overview of Metabolism

Overview of Metabolism

Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Phase II Reactions: Methylation Reactions

Phase II Reactions: Methylation Reactions

Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...

Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...

Biosynthesis of Nucleic Acids

Biosynthesis of Nucleic Acids

Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...