「ナノリボン・センチネル」：グリオ伝達物質放出のリアルタイムイメージング用細胞表面アプタセンサー
Meirong Cui1,2,3, Chenxu Hu1,2,3, Yiyu Cheng1,2,3
1State Key Laboratory for Flexible Electronics (LoFE), Nanjing University of Posts and Telecommunications, Nanjing, China. iamjchao@njupt.edu.cn.
Abstract:
We report a cell-surface-anchored "Nanoribbon Sentinel", an aptasensor overcoming endocytosis for real-time, high-resolution imaging of ATP on live astrocytes. This design uniquely couples a non-internalizing scaffold with a fast-kinetic "signal-on" aptamer, enabling high temporal resolution. The sensor successfully captured the transient, localized release of ATP from mechanically stimulated cells, offering a powerful new tool to study neurochemical signaling.
さらに関連する動画
13:47TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
Published on: January 29, 2015
12:48Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters CNiFERs for Optical Detection of Neurotransmitters In Vivo
Published on: May 12, 2016
