エンジニアリングされた動脈、静脈、およびがん関連血栓性微小環境における血小板動態のリアルタイムイメージング

Thrombosis is a leading cause of cardiovascular morbidity and mortality, driven by platelet-mediated mechanisms common across distinct vascular environments. However, the dynamical behavior of platelets during thrombogenesis remains poorly understood due to the lack of a comprehensive analytical framework. Here we present a physiologically relevant, imaging-integrated thrombosis-on-chip platform that enables real-time quantitative analysis of platelet dynamics during thrombogenesis under arterial, venous, and cancer-associated conditions. The system incorporates endothelialized 3D-printed vascular geometries into a closed-loop whole-blood perfusion circuit that replicates native hemodynamic and cellular microenvironments. Multimodal imaging captures the spatiotemporal evolution of thrombus formation and shows how hydrodynamic forces, endothelial dysfunction, and tumor-derived factors drive distinct thrombotic signatures. Notably, platelet-endothelium adhesion and circulating platelet aggregation are identified as mechanistically distinct yet closely linked processes, each uniquely modulated by vascular context. This platform offers a robust framework for dissecting thrombogenesis and advancing antithrombotic and cancer-associated thrombosis research.