骨髄炎病原体の時空間的マッピングが機能的勾配骨再生足場の設計を導く

Recurrent osteomyelitis remains a formidable challenge, with failure rates exceeding 25%. This study establishes a reproducible rabbit femoral model to systematically map the spatiotemporal pathogenesis of Staphylococcus aureus-induced osteomyelitis. Through longitudinal micro-CT and histo-morphometric analysis, we delineated a progressive pathological continuum: Acute trabecular destruction at 2 weeks advanced to subacute cortical devascularization by 4 weeks, culminating in chronic epiphyseal compromise with articular surface collapse at 6 weeks. A critical finding was the region-specific vulnerability to infection, with the diaphysis and condyle exhibiting distinct patterns of destruction. These quantitative pathoanatomical data directly inform a novel design paradigm for future bone regeneration strategies. We propose that effective therapeutic scaffolds must adopt a functionally graded approach, addressing the unique mechanical and biological requirements of each compromised region - specifically, tailored porosity gradients and architectural features for the diaphysis versus the condyle - rather than employing a uniform design. This work converts descriptive pathological progression into a set of predictive engineering criteria, providing a foundational platform for the future development of targeted infected-bone reconstruction.