新規コベントリーウイルス科ファージStaphylococcus pseudintermedius感染株のゲノムおよび機能的特徴解析
Patoo Withatanung1, Sukanya Apiratwarrasakul2, Pathomporn Sresuwadjarey2
1Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Abstract:
The rise of methicillin-resistant Staphylococcus pseudintermedius (MRSP), a multidrug-resistant zoonotic pathogen with strong biofilm-forming ability, highlights the urgent need for alternative therapies. Here, we report the isolation and comprehensive characterization of vB_SpsS_VL4 (VL4), a novel temperate phage obtained from sewage using a clinical MRSP isolate. VL4 genome (39,788 bp) contains 68 open reading frames (ORFs) with no virulence, resistance, or toxin genes and encodes lysis proteins with Cysteine, Histidine-dependent Amidohydrolase/Peptidase (CHAP) domains. Genomic and phylogenetic analyses classified VL4 as a new species within the Coventryvirus genus. Despite its temperate nature, VL4 demonstrated efficient replication (latent period 50 min; burst size 56 PFU per cell), remained viable at neutral pH and temperatures up to 37 °C, and significantly suppressed the host bacterial growth. Among 31 S. pseudintermedius clinical isolates, 27 (87%) strains were susceptible to VL4, while no activity was detected against Staphylococcus aureus. Moreover, VL4 inhibited biofilm formation and partially degraded mature MRSP biofilms. Collectively, these findings identify VL4 as a promising Coventryvirus phage with potential for future engineering and therapeutic development against difficult-to-treat MRSP infections.
関連する概念動画
DNA Bacteriophages
Lytic Cycle of Bacteriophages
Lysogenic Cycle of Bacteriophages
Viral Replication: Lysogenic Cycle
Bacterial Phylum Tenericutes
Bacterial Phylum Spirochaetes


