Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Infection01:20

Infection

11.6K
When a pathogen enters the body and reproduces, it can cause an infection, damage body cells, and cause illness symptoms that eventually lead to disease. Therefore, its prevention requires breaking the chain of infection.
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...
11.6K
Urinary Tract Infection II: Pathophysiology01:25

Urinary Tract Infection II: Pathophysiology

524
The pathophysiology of urinary tract infections (UTIs) encompasses several progressive stages, beginning with bacterial colonization and culminating in potential systemic complications if untreated. UTIs are primarily initiated by bacteria, such as Escherichia coli, which often originate from the gastrointestinal tract and migrate to the urinary system through the periurethral area. This migration can occur via several routes, including improper hygiene practices, sexual activity, or...
524
Cystic Fibrosis: Pathogenesis01:23

Cystic Fibrosis: Pathogenesis

676
Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...
676
Pneumonia II: Pathophysiology01:29

Pneumonia II: Pathophysiology

2.5K
The pathophysiology of pneumonia involves the following steps:
2.5K
Stages of Infection01:26

Stages of Infection

64.7K
Stages of infection describe what happens to a susceptible host once a pathogen invades the human body. The stages of infection are incubation, prodromal, illness, stage of decline, and convalescence. The incubation stage is the period from exposure to a pathogen until symptoms start. The infected person is unaware of impending illness as the pathogens grow and multiply within the body. The duration may vary depending on the type of infection. The incubation period of measles averages ten to...
64.7K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

2.6K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.6K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

The Concurrent Validity and Test-Retest Reliability of a Smartphone-Based Markerless System.

Sensors (Basel, Switzerland)·2026
Same author

Curated set of tool compounds to probe PYK2 and FAK signaling in Alzheimer's disease.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Comparative efficacy of neurofeedback and traditional mindfulness meditation on undergraduates' stress and mindfulness: A randomized controlled trial.

Journal of American college health : J of ACH·2026
Same author

MitoSAM-dependent lipoylation controls postnatal heart development via metabolic remodeling.

bioRxiv : the preprint server for biology·2026
Same author

Structure-Aware Compound-Protein Affinity Prediction via Graph Neural Networks with Group Lasso Regularization.

Computational and structural biotechnology journal·2026
Same author

Optimization and Characterization of SHIP1 Ligands for Cellular Target Engagement and Activity in Alzheimer's Disease Models.

Journal of medicinal chemistry·2026
Same journal

Evidence for progressive neurodegeneration in iatrogenic cerebral amyloid angiopathy.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Human brain connectome profiles mediate the relationship between pathology burden and clinical phenotypes in Alzheimer's disease.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Kat5 cKO mouse replicates biological domain signatures associated with Alzheimer's disease.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Evaluation of CSF and plasma tau species as fluid surrogate candidates for tau PET in prodromal to moderate Alzheimer's disease.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Associations of self-reported obstructive sleep apnea with cognition and dementia risk in cognitively unimpaired middle-aged adults.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same journal

Inflammation profiles in Alzheimer's disease relate to cognition and neurodegeneration.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
関連記事をすべて見る

関連する実験動画

Updated: Jan 8, 2026

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses
09:07

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

Published on: June 14, 2020

11.5K

基礎科学と病態生理

Joshua Kulas1, Angela K Haskell2, William Carter2

  • 1Indiana Biosciences Research Institute (IBRI), Indianapolis, IN, USA.

Alzheimer's & dementia : the journal of the Alzheimer's Association
|December 24, 2025
PubMed
まとめ
この要約は機械生成です。

iPSC由来ミクログリア様細胞（iMG）が生成され、特徴づけられた。これらのiMGモデルは刺激に応答し、神経炎症およびアミロイド病理を標的とする抗体療法をテストするために使用できる。

キーワード:
iPSC由来ミクログリア様細胞神経炎症TREM2アミロイド病理抗体療法

さらに関連する動画

A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
13:45

A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia

Published on: September 21, 2019

6.0K
Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses
08:38

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

Published on: February 22, 2019

6.3K

関連する実験動画

Last Updated: Jan 8, 2026

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses
09:07

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

Published on: June 14, 2020

11.5K
A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
13:45

A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia

Published on: September 21, 2019

6.0K
Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses
08:38

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses

Published on: February 22, 2019

6.3K

科学分野:

  • 神経科学; 幹細胞生物学; 免疫学

背景:

  • iPSC技術により、in vitroでヒトミクログリア様細胞（iMG）を作成できるようになった。; 新規iPSCラインIBRI 104.GがiMG分化に利用された。; 本研究では、iMGモデルの機能的および生化学的特性を評価した。

研究 の 目的:

  • iPSC由来ミクログリア様細胞（iMG）を分化させ、特徴づけること。; ミエリンデブリおよびアミロイドβ（Aβ）オリゴマーのような免疫原性刺激に対するiMGの応答を評価すること。; 抗アミロイドおよびTREM2標的抗体がiMG生物学に及ぼす影響を調査すること。

主な方法:

  • IBRI 104.G iPSCラインのエピソームリプログラミングベクターを用いた作製。; マトリゲルコーティングプレート上でのiMG分化。; マウス脳からのミエリンデブリおよび組換えペプチドからのAβオリゴマーの調製。; CHO細胞形質導入によるTREM2およびAβ抗体の産生。; ミクログリアの形態および貪食能を分析するためのハイスループット蛍光イメージング。

主要な成果:

  • IBRI 104.G iPSCラインは多能性マーカーを発現した。分化したiMGは主要なミクログリアマーカー（PU.1、P2RY12R、TMEM119、TREM2）を発現した。; iMGは樹枝状形態を示し、炎症刺激によりアメーバ状になり、ミエリンデブリおよびAβの貪食能を示した。; TREM2抗体はミエリン貪食を減少させたが、Aβ抗体はiMGによるアミロイド取り込みを増強した。

結論:

  • IBRI 104.G iPSCラインは、ヒトミクログリアの特徴を持つミクログリア様細胞へ効率的に分化する。; iMGは、神経炎症の研究および脳の骨髄細胞を標的とする治療用抗体のテストのための有用なin vitroモデルとして機能する。