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Updated: Jan 8, 2026

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield

Published on: January 7, 2019

リグニン由来モノマー芳香族エーテルの位置選択的芳香族O-脱メチル化のための工学的P450BM3ペルオキシゲナーゼシステム

Sijia Zhao^1,2, Di Yan^1,3, Osami Shoji⁴

¹State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.

Journal of agricultural and food chemistry

|December 24, 2025

Abstract:

Regioselective O-demethylation of lignin-derived aryl ethers is a key yet challenging step in lignin valorization, governing the efficient conversion of lignin into high-value aromatic chemicals. Here, we report an artificial peroxygenase system that integrates engineered P450BM3 with glucose oxidase (GOx) and a dual-functional small molecule (DFSM) to enable efficient O-demethylation driven by in situ H₂O₂ generation. This strategy circumvents the dependence on costly NADPH and improves catalytic sustainability and operational robustness. The engineered F87A/T268D/A328F mutant achieved efficient double O-demethylation of syringol with a turnover number (TON) of 583 ± 7, while the F87A/V78A mutant exhibited a 68.2-fold enhancement in catalytic efficiency toward anisole (TON = 341 ± 8) compared to wild-type P450BM3. Precise regulation of oxidative catalysis and H₂O₂ supply afforded high efficiency, selectivity, and stability. This work establishes a scalable and environmentally benign biocatalytic platform for upgrading lignin-derived aromatic monomers, advancing sustainable biomass utilization.

キーワード:

O-脱メチル化 P450BM3 リグニン

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Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues

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