関連する実験動画

Updated: Jan 8, 2026

Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy

Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy

Published on: November 29, 2017

環境および生体システムにおけるHClOの高感度検出と視覚化のための新規蛍光プローブの開発

Mingshuo Ma¹, Bingxin Zhu², Shihua Yu²

¹Center of Characterization and Analysis, Jilin University of Chemical Technology, Jilin, People's Republic of China. jiangzxd@163.com.

Physical chemistry chemical physics : PCCP

|December 24, 2025

Abstract:

A novel coumarin-based fluorescent probe was designed and synthesized for highly sensitive detection of HClO. It ingeniously integrates coumarin as the fluorophore and formamide as a HClO-specific responsive site. When exposed to HClO, it exhibited a significant fluorescence decrease response characterized by a rapid response time (within 2 min), a high sensitivity (4.96 nM), and excellent selectivity and anti-interference performance. It successfully imaged exogenous and endogenous HClO in living cells and zebrafish. In addition, it has also demonstrated its practicality and accuracy in actual water sample testing. All the experimental results confirm that this probe has a very broad application prospect.

キーワード:

蛍光プローブ次亜塩素酸化学センサー分析化学イメージング

さらに関連する動画

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Published on: March 9, 2017

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

関連する概念動画

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...