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Updated: May 5, 2026

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In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice
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近赤外線IIウィンドウにおけるカルシウムイメージング
Danyang Xu1,2, Zhisheng Wu1, Hui Yang3
1Department of Electrical and Electronic Engineering, The University of Hong Kong, Kowloon, Hong Kong SAR 999077, China.
Journal of the American Chemical Society
|December 24, 2025
まとめ
研究者は細菌のタンパク質を用いた新しい近赤外線II (NIR-II) カルシウム画像技術を開発しました. この方法は,生きた哺乳類におけるカルシウムイオン (Ca2+) ダイナミクスの高感度,深層組織イメージングを可能にし,腫瘍治療の評価に役立ちます.
科学分野:
- バイオメディカル光学
- 分子イメージング
- バイオテクノロジー
背景:
- 生きている哺乳類の深層組織における伝統的なカルシウムイオン (Ca2+) 画像は,可視波長 (400~750 nm) の光の散乱によって制限されます.
- 高度な感度と解像度は,非侵襲的なイメージング,特にダイナミックな生物学的プロセスと治療反応のモニタリングに不可欠です.
研究 の 目的:
- 生きた哺乳類の非侵襲的な深層組織画像化のための新しい近赤外線II (NIR-II) 窓カルシウム画像技術を開発する.
- NIR-IIスペクトル (>1000 nm) で Ca2+インジケーター特性を向上させるための*Thermochromatium tepidum*の天然タンパク質を調査する.
- 腫瘍治療の有効性を縦断的に評価するために,このNIR-II Ca2+画像の有用性を実証する.
主な方法:
- 熱染色体 (Thermochromatium tepidum) から派生した新型,高度に光学的に安定した光タンパク質の探査と特徴付け
- 培養された哺乳類の細胞で細胞内Ca2+イメージングのためにタンパク質を使用し,可視Ca2+指標と感度を比較した.
- 生きた哺乳類の無傷な腫瘍の*in vivo* NIR- II Ca2+イメージングを行い,異なる治療戦略に対する反応を監視した.
主要な成果:
- 開発された細菌のタンパク質は,哺乳類の細胞内Ca2+反応の敏感なNIR-IIイメージングを可能にします.
- NIR-II波長を用いた高感度,高解像度,高コントラストの*in vivo*腫瘍におけるCa2+トランジエントのイメージングを達成した.
- 非侵襲的深層組織画像を用いて腫瘍治療の有効性を長期的に評価する可能性を実証した.
結論:
- 新しい NIR-II 光タンパク質は,高度な深層組織カルシウムイメージングを1000 nm 以外で可能にします.
- この技術は生きた哺乳類の生物学的プロセスと 治療反応の非侵襲的なモニタリングに 有望な機会を提供します
- 様々な生物医学的な応用における治療効果の縦断的な評価の可能性を開きます.
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