ColorSTEM：ラベル付けされた生物学的標本の多色電子顕微鏡検査のための簡単で便利なアプローチ
Abstract:
A new method for Multicolor Electron Microscopy (EM) using scanning transmission EM, ColorSTEM has been developed to increase the sensitivity, instrument availability and ease of use compared to that previously developed using electron energy loss spectroscopy and energy filtered transmission EM. Both methods paint multiple subcellular complexes with different colors, enabled by the localized and targeted deposition of diaminobenzidine (DAB) conjugated to different metals, which provides the differentiating signal from each other. With ColorSTEM, elastic scattering that is dependent upon atomic weight (Z-contrast) has far greater signals. Using deposited DAB with copper or gold to label subcellular components, Z-contrast imaging generates a pseudo-elemental map, resulting from differences in the grayscale of the conventional STEM images.
関連する概念動画
01:03Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
01:20Immunogold Electron Microscopy
01:24Simple Staining Technique