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Updated: Jan 7, 2026

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Published on: October 29, 2015

二重標的、長循環、インテリジェント応答性抗ウイルス剤がウイルス侵入と複製をブロックすることによりSARS-CoV-2を抑制

Jingyang Zhao^1,2, Peng Ye^2,3, Huatai Zhu^2,3

¹State Key Laboratory of Biopharmaceutical Preparation and Delivery, Institute of Process Engineering, China Academy of Sciences, Beijing 100190, China.

Bioconjugate chemistry

|December 25, 2025

Abstract:

Various natural drugs such as glycyrrhizic acid (GA), hesperidin (Hes), and baicalin (Bai) exhibit anti-SARS-CoV-2 potential but suffer from poor water solubility, short half-life, and low binding capacity to viral targets. Hence, a dual-targeted, long-circulating, ROS-responsive, and broad-spectrum antiviral inhibitor (mPAGHB) is designed through a strategy of "polymer-drug linkage". The mPAGHB suppresses SARS-CoV-2 through two "lines of defense", including extracellular inhibition of the "spike protein-angiotensin converting enzyme 2 (ACE2)" process and intracellular inhibition of the enzymatic activity of major protease. It exhibits higher affinity for spike protein (K_D = 4.95 × 10^-2 μM), excellent inhibition of M^pro activity (76.27 ± 5.94% inhibition), and improved inhibition for pseudovirus of SARS-CoV-2 and Omicron variants in cellular and animal assays. In addition, as an efficient generic strategy, the "polymer-drug linkage" strategy facilitates the rapid construction of antiviral inhibitors based on natural active ingredients for the outbreaks of viral public infections.

キーワード:

抗ウイルス剤 SARS-CoV-2 スパイクタンパク質 Mpro ポリマー薬コンジュゲートドラッグデリバリーナノテクノロジー生化学薬理学

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Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

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