ターゲット液体クロマトグラフィー三重四重極質量分析法を用いた死後経過時間推定のためのペプチド比
Jenna Ireland1, Lana Brockbals1,2, Dennis McNevin1
1Centre for Forensic Sciences, School of Mathematical and Physical Sciences, University of Technology Sydney, Sydney, NSW, 2007, Australia.
International journal of legal medicine
|December 25, 2025
まとめ
本研究では、筋肉組織を用いた死後経過時間（PMI）推定のためのターゲットプロテオミクス法を最適化した。開発された液体クロマトグラフィー-三重四重極質量分析法（LC-QQQ）アプローチは、日常的な法科学的ケースワークに有望である。
科学分野:
- Forensic Science; Biochemistry; Analytical Chemistry
背景:
- Postmortem interval (PMI) estimation is crucial in forensic investigations but faces challenges, especially in later decomposition stages.; Current PMI methods have limitations; proteomics offers a more objective approach.; Previous research identified 19 peptide targets in muscle tissue correlated with decomposition time.
研究 の 目的:
- To optimize the detection of 19 previously identified peptide targets using a targeted liquid chromatography-triple quadruple mass spectrometry (LC-QQQ) method.; To assess the reproducibility and applicability of this targeted LC-QQQ method for forensic casework.; To conduct a longitudinal analysis of peptide targets in authentic human samples over 14 days postmortem.
主な方法:
- Development and optimization of a targeted LC-QQQ method for 19 specific peptide targets.; Analysis of human muscle tissue samples over a 14-day postmortem period.; Data analysis including generation of peptide ratios and log2 transformation.
主要な成果:
- 17 out of 19 peptide targets were optimized for reproducible detection.; The targeted LC-QQQ method yielded interpretable results for most targets over the 14-day period.; Two previously proposed peptide ratios demonstrated linear trends after data transformation.
結論:
- A targeted LC-QQQ method is a viable system for detecting PMI peptide targets in human postmortem muscle tissue.; The optimized method shows potential for validation and application in routine forensic casework.; This proteomics-based approach offers a more objective tool for PMI estimation.
