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関連する概念動画

Preclinical Development: Overview01:28

Preclinical Development: Overview

5.7K
Preclinical development consists of a series of tests that ensure the safety and efficacy of a new therapeutic compound before it is tested in humans. There are four main phases to this process. First, safety pharmacology tests are conducted to ensure the drug does not produce any acutely harmful effects. These tests examine parameters such as bronchoconstriction, cardiac dysrhythmias, blood pressure changes, and ataxia. Next, preliminary toxicological testing is performed to determine the...
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Clinical Trials: Overview01:11

Clinical Trials: Overview

4.5K
Clinical development focuses on how the drug will interact with the human body and encompasses four key phases of clinical trials, each serving a specific purpose in assessing the safety and effectiveness of new drugs. These phases overlap and build upon one another. Phase I involves a small group of healthy volunteers (typically 20-80 individuals) or, in cases where significant toxicity is expected, patients with the targeted disease, such as cancer or AIDS. The volunteers are tested for...
4.5K
Drug Discovery: Overview01:26

Drug Discovery: Overview

10.9K
Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
10.9K
Drug Administration and Therapy Phases: Overview01:26

Drug Administration and Therapy Phases: Overview

1.1K
Drugs, the chemical agents used in diagnosing, treating, or preventing diseases, undergo a four-phase process of development: pharmaceutic, pharmacokinetics, pharmacodynamics, and therapeutic.
The pharmaceutical phase focuses on leveraging the physicochemical properties of the drug to design and manufacture an effective product. Variants include orally administered tablets or capsules, topical creams or ointments, and parenteral-delivery solutions or emulsions.
The pharmacokinetic phase...
1.1K
In Vitro Drug Release Testing: Overview, Development and Validation01:10

In Vitro Drug Release Testing: Overview, Development and Validation

279
In vitro dissolution and drug release tests assess how quickly and how much of a drug is released from its dosage form into an aqueous medium under standardized laboratory conditions. These tests are essential tools in pharmaceutical development and quality assurance, offering insight into the drug's performance before clinical use.During formulation development, dissolution testing identifies incomplete or inconsistent drug release issues. It also supports decisions on selecting the optimal...
279
Drug Regulation01:25

Drug Regulation

2.7K
Drug regulation encompasses the management of drug usage by evaluating its safety and efficacy through assessments conducted by regulatory authorities. Regrettably, the history of drug regulation is marred by several catastrophic events. One such incident is the Elixir Sulfanilamide tragedy, in which the toxic compound diethyl glycol was included in a sweet-tasting medication, leading to numerous fatalities. This event prompted the enactment of the Food, Drug, and Cosmetic Act in 1938. Under...
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Drug Repurposing Hypothesis Generation Using the "RE:fine Drugs" System
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薬物開発

Jai Rudra1, Dev Patel2

  • 1Washington University in St. Louis St. Louis, St. Louis, MO, USA.

Alzheimer's & dementia : the journal of the Alzheimer's Association
|December 26, 2025
PubMed
まとめ

オリゴペプチドのキラリティは、フィブリルや2次元βシートなどの異なる構造への自己集合に影響を与える。これらのペプチド構造を理解することは、神経変性疾患の新しい治療法を開発する鍵となる。

科学分野:

  • 生化学
  • 材料科学

背景:

  • 交互の極性および非極性構造を持つオリゴペプチドの自己集合は、アルツハイマー病などの神経変性疾患の治療の可能性を提供する。
  • ペプチドの自己集合は、脂質二重層やDNAと同様の分子組織力によって支配され、キラリティに決定的に依存する。
  • キラリティ、アミノ酸側鎖の空間配置は、ペプチド自己集合の適応性の性質を決定する。

研究 の 目的:

  • L-およびD-アミノ酸を使用したKFE12ペプチドアナログの自己集合におけるキラリティの役割を調査すること。
  • KFE12ペプチドの8つの脱ブロックヘテロキラルアナログの構造特性を調べること。
  • 電子顕微鏡（EM）を使用してペプチド集合体を視覚化すること。

主な方法:

  • 固相ペプチド合成によるKFE12アナログの合成。
  • ジクロロメタン洗浄および開裂反応を使用したペプチドの精製。
  • ペプチドサンプルの溶解、グリッドへの適用、染色、JOEL 2100F電子顕微鏡での画像取得によるEMサンプルの調製。

主要な成果:

  • KFE12アナログは、キラリティに基づいて異なる自己集合挙動を示した。
  • あるアナログ、DLDは、約20nm幅のフィブリルを形成し、約200nmのピッチで観察可能なねじれを示した。
キーワード:
オリゴペプチド自己集合神経変性疾患キラリティペプチド構造治療KFE12アナログフィブリルβシートアルツハイマー病

さらに関連する動画

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
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Developmental Toxicity Assay Based on Real-Time Monitoring of Fibroblast Growth Factor Signal Disruption in Human Induced Pluripotent Stem Cells
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Developmental Toxicity Assay Based on Real-Time Monitoring of Fibroblast Growth Factor Signal Disruption in Human Induced Pluripotent Stem Cells

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関連する実験動画

Last Updated: Jan 7, 2026

Drug Repurposing Hypothesis Generation Using the "RE:fine Drugs" System
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Drug Repurposing Hypothesis Generation Using the "RE:fine Drugs" System

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In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
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In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

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451
  • 別のアナログ、DLLは、モノマーの急速な枯渇により、2次元βシートとより小さな凝集体を形成する不均一な集合を示した。

    • 結論:

    • KFE12ペプチドにおけるアミノ酸側鎖の位置は、結果として得られる構造と特性に大きく影響する。
    • 分光分析（赤外線および円二色性）は、エナンチオマーのユニークな構造と折り畳みパターンを確認し、異なる特性につながった。
    • 自己集合メカニズムとその治療的応用を完全に解明するには、高度な画像処理を含むさらなる研究が必要である。