Metabolite interactions mediate beneficial alliances between Bacillus and Trichoderma for effective Fusarium wilt...

科学分野:

• Microbiology
• Soil Science
• Plant Pathology

背景:

• Bacteria-Fungi Interactions are vital for soil health and plant disease management.
• Bacillus and Trichoderma species show antagonism in vitro but positive correlation in soil.
• These microbes are linked to suppressing Fusarium oxysporum and promoting plant growth.

研究 の 目的:

• To investigate the cross-kingdom interactions between Bacillus velezensis and Trichoderma guizhouense.
• To understand the molecular mechanisms underlying their beneficial interactions in a model soil community.
• To elucidate how these interactions contribute to plant protection against Fusarium oxysporum.

主な方法:

• Constructing artificial model communities of Bacillus velezensis and Trichoderma guizhouense.
• Utilizing transcriptomic profiling to analyze gene expression changes.
• Chemical analysis to identify key metabolites involved in the interactions.

主要な成果:

• Bacillus velezensis activates antimicrobial secondary metabolite production in response to Trichoderma guizhouense.
• Surfactin from B. velezensis induces T22azaphilone production in T. guizhouense, mitigating oxidative stress.
• Both surfactin and T22azaphilone aid in the survival of B. velezensis and T. guizhouense against Fusarium oxysporum.
• Trichoderma guizhouense degrades fusaric acid, a growth inhibitor secreted by Fusarium oxysporum.

結論:

• Metabolite-mediated interactions between Bacillus and Trichoderma create beneficial alliances.
• These alliances enhance the resilience of soil microorganisms against pathogens.
• The findings highlight a novel mechanism for microbial-based plant protection against soil-borne diseases.