未処理ヒト血清中の連続乳酸測定の進歩：低コスト3Dプリントマイクロ流体セルに結合したマルチポリマーベースのアンペロメトリックバイオセンサー

科学分野:

• Electrochemistry; Biosensing; Biomedical Engineering

背景:

• Enzyme saturation is a significant challenge in lactate biosensing due to high analyte concentrations in biological samples.; Developing stable and sensitive biosensors is crucial for accurate lactate monitoring in clinical diagnostics.

研究 の 目的:

• To fabricate a robust amperometric biosensor for lactate determination that minimizes enzyme saturation.; To integrate the biosensor into a 3D-printed microfluidic cell for real-time monitoring in flow systems.

主な方法:

• Fabrication of an amperometric biosensor using a Sonogel–carbon transducer with immobilized lactate oxidase and multipolymer coatings.; Development of a polyethylene terephthalate glycol (PETG) microfluidic cell via 3D printing.; Testing biosensor performance with various interferents and in real human serum samples.

主要な成果:

• The biosensor exhibited good sensitivity (1.74 µA mM⁻¹ cm⁻²) within a physiological linear range (0.2–20 mM).; Negligible interference was observed from common electroactive species found in biological fluids.; The 3D-printed microfluidic system demonstrated reliable lactate monitoring in flow, correlating well with standard methods.

結論:

• The proposed microfluidic amperometric biosensor effectively addresses enzyme saturation for improved lactate monitoring.; The system shows potential as a reliable, real-time tool for biomedical applications, particularly in analyzing human serum.