ヒト胆嚢上皮細胞由来肝細胞を化学的に定義されたアプローチで生成するためのプロトコル

The large-scale production of functional hepatocytes is critical for liver disease treatment, disease modeling, and drug development. Here, we present a protocol for isolating, expanding, and maintaining human gallbladder epithelial cells (hGBECs). We describe steps for characterizing hGBECs and their differentiation into hepatocytes. We then detail procedures to assess the functionality of the derived hepatocytes using key hepatic function assays. For complete details on the use and execution of this protocol, please refer to Chen et al.¹.