光シートイメージングと2D空間多重化による3Dガイド下切断および深層細胞表現型解析プロトコル
Kevin Bigott1, Victoria H Schoppel1, Manuel Martinez-Osuna1
1Miltenyi Biotec B.V. & Co. KG, 51429 Bergisch Gladbach, Germany.
Abstract:
Spatial analysis of cells and their microenvironment within tissues enhances our understanding of biological processes. Ideally, a broad range of biomolecules should be analyzed in large 3D tissue specimens at subcellular resolution. Here, we present a protocol to identify and extract target sections from previously cleared tissues. We describe steps for combining 3D light sheet imaging and subsequent 3D-guided deep cell phenotyping via multi-cyclic 2D microscopy.
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