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Updated: Jan 7, 2026

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

Published on: October 31, 2019

使用済みモーターオイルの混合細菌群集による生分解ポテンシャル：単一チャンバー微生物燃料電池を用いた最適化、乳化活性、電気化学的およびメタゲノム解析

Ebtehag A E Sakr¹, Nahla M Mansour², Hanaa M Sabaa³

¹Botany Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt. Ebtehag.Abdelfattah@women.asu.edu.eg.

Microbial cell factories

|December 28, 2025

要旨

Background:

Used motor oil (UMO) is a dangerous environmental pollutant that needs to be treated effectively. This work introduces a novel approach for producing bioelectricity and UMO biodegradation simultaneously in a single-chamber microbial fuel cell (SCMFC) using native mixed bacterial cultures.

Results:

Under certain conditions (2% oil, 1% peptone, 4% inoculum, 21 days), the optimized bacterial culture degraded UMO by about 80%. Through bioelectrochemical studies, a maximum voltage of 257 mV and a power density of 36.6 mW/m² were demonstrated, showing a strong correlation between UMO removal and electricity generation. Moreover, metagenomic data showed that Firmicutes, particularly Bacillus, dominated the biofilm at roughly 65%. Fourier Transform Infrared (FTIR) and Gas Chromatography-Mass Spectroscopy (GC-MS) verified the breakdown of complex hydrocarbon molecules, highlighting their crucial role in UMO biodegradation and bioenergy production. The effective elimination of UMOs and simultaneous power generation, supported by metagenomic and biochemical tests, showed the microbial activity and hydrocarbon breakdown.

Conclusions:

The results suggest SCMFC technology as a sustainable solution for managing petroleum waste while producing renewable energy.

キーワード:

FTIR GC–MS メタゲノム解析最適化電力密度 SCMFC 使用済みモーターオイル

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Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

Published on: December 7, 2021

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Published on: October 2, 2012

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