Cell2Read:ヒト細胞懸濁液からシーケンシング準備済みDNAライブラリを生成するための自動ワークフロー
Kathryn Whitehead1, Sarah Planchak1, Trinity Williams1
1Center for Biomedical Engineering, School of Engineering, Brown University, Providence, RI 02912, United States.
Biology methods & protocols
|December 30, 2025
まとめ
Cell2Readは、細胞溶解および次世代シーケンシング(NGS)用サンプル調製を自動化し、わずか1500個の細胞から高品質DNA分析を可能にします。この新しい方法は、ゲノム研究および診断に不可欠な、多様な細胞タイプに対して再現性の高い結果を保証します。
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関連する概念動画
Next-generation Sequencing
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
