神経細胞とがん細胞の相互作用試験用デバイスの作製と利用プロトコル

The device for axon and cancer cell interaction testing (DACIT) mimics neuron-cancer interactions by compartmentalizing neuron soma and axons. Here, we present a protocol to design, fabricate, load, and image the DACIT. We describe steps for DACIT master generation, smooth-on mold replication, and DACIT fabrication. We then detail procedures for cell loading in 2D and 3D settings. This protocol also includes custom 3D-printed imaging holders. For complete details on the use and execution of this protocol, please refer to Velazquez-Quesada et al.¹.