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関連する概念動画

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.6K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.6K
Labeling DNA Probes03:31

Labeling DNA Probes

9.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.2K

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関連する実験動画

Updated: Jan 7, 2026

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
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Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

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ローダミン結合タンパク質の迅速,明快,および可逆の光ラベル付け

Julian Kompa1, Lars J Dornfeld1, Nicola Porzberg1

  • 1Department of Chemical Biology, Max Planck Institute for Medical Research, Heidelberg 69120, Germany.

Journal of the American Chemical Society
|December 30, 2025
PubMed
まとめ

No abstract available in PubMed .

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Last Updated: Jan 7, 2026

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