関連する実験動画

Updated: May 5, 2026

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Published on: November 13, 2011

in vivo AGO-APPによるマウス脳における細胞種およびコンパートメント特異的なmiRNAプロファイリング

Surbhi Kapoor¹, Andrea Erni¹, Francesca Vincenzi¹

¹Aix-Marseille Université, Centre National pour la Recherche Scientifique (CNRS), Institut de Biologie du Développement de Marseille (IBDM), Marseille, France.

Cell reports methods

|December 30, 2025

Abstract:

AGO-APP through the expression of the T6B peptide permits the isolation of Ago-bound microRNAs (miRNAs). Here, we present the generation and characterization of two transgenic mouse lines that enable AGO-APP to be performed in vivo. First, we generated mice for CRE-dependent T6B expression throughout the cell. Using this line, we performed AGO affinity purification (AGO-APP) in olfactory bulb (OB) inhibitory interneurons and cerebral cortex excitatory neurons. Bioinformatic analysis validated the high reproducibility of the approach. It also demonstrated that, despite global miRNome conservation between the two cell types, a set of miRNAs, including the miR-200 family and the miR-183/96/182 cluster, is massively enriched in OB interneurons, which aligns with previous observations. In the second mouse line, T6B is fused to the postsynaptic protein PSD95. Isolation of T6B-PSD95 fractions from OB and cortical neurons identified specific sets of postsynapse-enriched miRNAs. Gene ontology analyses confirmed that these miRNAs preferentially target mRNAs related to synaptic functions.

キーワード:

アルゴノート CP: 細胞生物学ダイサー T6B TNRC6 皮質グルタミン酸作動性ニューロン miRNA 嗅球介在ニューロンシナプス後部

さらに関連する動画

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

関連する概念動画

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...