中国初生植被覆盖受损夯土城墙的MICP修复技术研究

As a typical representative of soft capping, primary vegetation capping has both protective and destructive effects on earthen city walls. Addressing its detrimental aspects constitutes the central challenge of this project. Because the integration of MICP technology with plants offered advantages, including soil solidification, erosion resistance, and resilience to dry-wet cycles and freeze-thaw cycles, the application of MICP technology to root-soil composites was proposed as a potential solution. Employing a combined approach of RF-RFE-CV modeling and microscopic imaging on laboratory samples from the Western City Wall of the Jinyang Ancient City in Taiyuan, Shanxi Province, China, key factors and characteristics in the mineralization process of Sporosarcina pasteurii were quantified and observed systematically to define the optimal pathway for enhancing urease activity and calcite yield. The conclusions were as follows. The urease activity of Sporosarcina pasteurii was primarily regulated by three key parameters with bacterial concentration, pH value, and the intensity of urease activity, which required stage-specific dynamic control throughout the growth cycle. Bacterial concentration consistently emerged as a high-importance feature across multiple time points, with peak effectiveness observed at 24 h (1.127). pH value remained a highly influential parameter across several time points, exhibiting maximum impact at around 8 h (1.566). With the intensity of urease activity, pH exerted a pronounced influence during the early cultivation stage, whereas inoculation volume gained increasing importance after 12 h. To achieve maximum urease activity, the use of CASO AGAR Medium 220 and the following optimized culture conditions was recommended: an activation culture time of 27 h, an inoculation age of 16 h, an inoculation volume of 1%, a culture temperature of 32 °C, an initial pH of 8, and an oscillation speed of 170 r/min. Furthermore, to maximize the yield of CaCO₃ in output and the yield of calcite in CaCO₃, the following conditions and procedures were recommended: a ratio of urea concentration to Ca²⁺ concentration of 1 M:1.3 M, using the premix method of Sporosarcina pasteurii, quiescent reaction, undisturbed filtration, and drying at room-temperature in the shade environment.