感染創傷治癒促進のための血小板豊富血漿を強化した多機能キトサンベースヒドロゲルのワンステップ治療戦略

Infected wounds cannot be effectively resolved by a single-function dressing owing to their multifactorial microenvironment. Herein, we fabricated a platelet-rich-plasma (PRP), quaternized lipoic-acid-modified chitosan hydrogel (QLCP) by dispersing PRP within a ultraviolet-triggered disulfide-crosslinked chitosan network bearing permanent cationic charges. Benefiting from the redox-responsive disulfide bonds and quaternization, the hydrogel not only forms an injectable, conformal, moisture-retaining barrier, but also provides on-demand reactive oxygen species scavenging together with sustained release of PRP-derived growth factors. The QLCP matrix efficiently diminished oxidative stress and nearly eradicated E. coli/S. aureus in vitro, while PRP promoted fibroblast proliferation and migration. In a rat full-thickness infected wound model, QLCP markedly accelerated wound closure, enhanced re-epithelialization, and reduced collagen disorganization. Both in vitro and in vivo results demonstrate that this hydrogel platform mitigates infection, depresses inflammation/oxidative injury, and facilitates tissue regeneration through a synergistic combination of physical sealing and exudate management, antibacterial/antioxidative activity, and bioactive factor delivery, highlighting its strong application potential for infected-wound therapy.