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Updated: May 6, 2026

mRNA Interactome Capture from Plant Protoplasts
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野生型トマトの葉片からSlREC2を免疫親和染色法と免疫降水法を用いて浄化

Lingling Zhu1, Robert M Larkin1

  • 1National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.

Frontiers in plant science
|January 2, 2026
PubMed
まとめ

No abstract available in PubMed .

キーワード:
スレック2免疫関係クロマトグラフィー免疫プレシピテーションマルチサブユニットタンパク質複合体タンパク質の浄化クロロプラストの減少

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Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
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