Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

DNA Bacteriophages01:26

DNA Bacteriophages

790
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
790
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

77.4K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
77.4K
Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

668
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
668
Prokaryotic Gene Structure and Organization01:28

Prokaryotic Gene Structure and Organization

1.8K
Prokaryotic genomes exhibit a streamlined organization of coding and non-coding regions essential for gene expression and protein synthesis. While coding regions contain the genetic instructions for proteins or functional RNAs, non-coding regions regulate the precise transcription and translation of these genes.Coding Regions: Proteins and RNAsThe primary coding regions, known as structural genes, include sequences transcribed into messenger RNA (mRNA) and ultimately translated into...
1.8K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

20.5K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
20.5K
Bacterial Phylum Tenericutes01:24

Bacterial Phylum Tenericutes

441
The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
441

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Decoding Viral Dark Matter: Metagenomic Prokaryotic Virus Characterization With Pharokka, Phold, and Phynteny.

Current protocols·2026
Same author

Reciprocal adaptation is critical in enhancing S. aureus and P. aeruginosa biofilm biomass.

Archives of microbiology·2026
Same author

Computational prediction resolves thousands of homooligomeric phage protein structures.

bioRxiv : the preprint server for biology·2026
Same author

Structural motif search across the protein universe with Folddisco.

Nature biotechnology·2026
Same author

Reply to Stratified Anti-Biofilm Therapy: Translating Staphylococcal Exoprotein Toxicity Hierarchies Into Clinical Practice.

International forum of allergy & rhinology·2026
Same author

Gallium Protoporphyrin and Lipid Crystalline Nanoparticles Promote the Activity of Ampicillin against MRSA.

ACS infectious diseases·2026

関連する実験動画

Updated: Jan 13, 2026

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
09:40

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

12.7K

Pholdを用いたタンパク質構造情報に基づくバクテリオファージゲノムアノテーション

George Bouras1,2, Susanna R Grigson3, Milot Mirdita4

  • 1Adelaide Medical School, Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, 5005, Australia.

Nucleic acids research
|January 7, 2026
PubMed
まとめ

タンパク質構造を利用した新しいツールであるPholdは、バクテリオファージ（ファージ）ゲノムアノテーションを強化します。シーケンスベースの方法と比較して、機能予測の精度と速度が向上し、ファージ療法開発に役立ちます。

キーワード:
バクテリオファージゲノムアノテーション構造ベースのアノテーションファージ療法タンパク質構造計算生物学ウイルス学

さらに関連する動画

An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

6.9K
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
08:31

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

11.5K

関連する実験動画

Last Updated: Jan 13, 2026

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
09:40

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

12.7K
An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

6.9K
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
08:31

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

11.5K

科学分野:

  • 計算生物学
  • 構造生物学
  • ウイルス学

背景:

  • バクテリオファージ（ファージ）ゲノムのアノテーションは、ウイルスの機能と治療の可能性を理解するために不可欠です。
  • 現在の方法は配列相同性に依存していますが、これは精度と感度を制限する可能性があります。

研究 の 目的:

  • タンパク質構造情報を活用した新しいアノテーションフレームワークであるPholdを導入します。
  • ファージゲノムの機能アノテーションの精度、速度、スケーラビリティを向上させます。

主な方法:

  • Pholdは、ProstT5タンパク質言語モデルとFoldseek構造アラインメントツールを統合します。
  • 機能ラベル付きの136万以上の予測ファージタンパク質構造のデータベースを利用します。
  • 既存のシーケンスベースの相同性アプローチと比較してベンチマークされています。

主要な成果:

  • Pholdは、シーケンスベースの方法と比較して、優れた機能アノテーション感度を示します。
  • 平均的なファージゲノムの50％以上の遺伝子と、古細菌ウイルスゲノムの40％の遺伝子の一貫したアノテーションを達成します。
  • ファージタンパク質は、特に核酸代謝と酵素機能に関与するタンパク質において、生命の木全体にわたる保存タンパク質との構造相同性を示すことがよくあります。

結論:

  • Pholdは、ファージゲノムアノテーションのための堅牢で効率的なソリューションを提供します。
  • その構造ベースのアプローチは、機能予測を強化し、ファージ生物学の理解を広げます。
  • このツールは、ファージベースの治療法と研究の進歩をサポートします。