乳がん細胞の非線形ダイナミクスと機械的特性に振動刺激周波数がどのように影響するか？

Understanding the mechanical properties of cells is crucial for gaining insights into their physiological and pathological states. This study focuses on the mechanical behavior of human mammary epithelial cells (MCF-10) and human breast cancer cells (MCF-7), emphasizing mechanical frequencies, finite element modeling (FEM), and nonlinear dynamics of the cells.

Cells were cultured and subjected to mechanical testing using Atomic Force Microscopy (AFM) and Magnetic Tweezer Cytometry (MTC). The elastic and viscoelastic properties were analyzed, and FEMs were developed to simulate cell behavior under various mechanical stimuli. The nonlinear dynamic behavior was examined using the Duffing model, and chaos was assessed using the Largest Lyapunov exponent (LLE).

MCF-10 cells exhibited higher stiffness than MCF-7 cells. The mechanical frequencies of both cell types were determined, and significant differences were observed at higher frequencies. FEM simulations provided detailed insights into the stress distribution and deformation patterns within cells. The nonlinear analysis revealed chaotic behavior at specific frequencies, particularly in the range of 22-36 kHz.

Identifying the mechanical frequencies and responses of cancer cells, including their nonlinear and chaotic behaviors, can inform the development of noninvasive therapeutic strategies. Further research is required to refine these models and explore the potential of mechanical forces in cancer treatment.