容易に入手可能な酵素応答性蛍光色素を用いた酵素および光による重合制御

Kanta Ogura1, Masahiko Nakamoto1,2, Michiya Matsusaki1

  • 1Division of Applied Chemistry, Graduate School of Engineering, The University of Osaka, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan. m-nakamoto@chem.eng.osaka-u.ac.jp.

Chemical communications (Cambridge, England)
|January 7, 2026
PubMed

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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