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Updated: Jan 13, 2026

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Published on: February 12, 2019

Schizophyllum communeの生育における細胞壁多糖類シゾフィランの機能

Fleur E L Kleijburg¹, Ella M Schunselaar¹, Adil A Safeer²

¹Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.

Cell surface (Amsterdam, Netherlands)

|January 8, 2026

Abstract:

Schizophyllan, a β-(1,3)(1,6)-glucan, is part of the cell wall of the mushroom-forming fungus Schizophyllum commune and is also released into the culture medium. It has various commercial applications but the natural function of schizophyllan during growth of S. commune is largely unknown. The S. commune strain H4-8A was grown on minimal medium (MM-N) and medium containing 10-fold more KH₂PO₄/K₂HPO₄ buffer (MM-NKP). The addition of extra buffer resulted in a 4.2-fold decrease in water-soluble schizophyllan and a 8.8-fold decrease in rigid schizophyllan in the cell wall. This decrease in schizophyllan was associated with a 3.7 fold lower tensile strength and a 2.5-fold higher elasticity of the mycelium. Moreover, spores of S. commune, as well as cells of Escherichia coli and Pseudomonas putida, showed increased survival against heat treatment and freeze-thawing, and had a longer shelf-life in the presence of schizophyllan. Also, schizophyllan can be metabolized by S. commune but the tested bacteria were unable to do so. Together, schizophyllan provides rigidity to the cell wall, protects S. commune against temperature stress, and can be used as an external carbon storage. It may also form a selective barrier around the hyphae, protecting S. commune against attack by bacteria.

キーワード:

細胞壁環境ストレス菌類シゾフィラン Schizophyllum commune β-(1,3)(1,6)-グルカン

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