カメリア種子油体のクルクミン負荷と多区画エマルションの製造：高圧ホモジナイゼーション前処理の影響

In this study, multi-compartment emulsion based on camellia seed oil bodies (CSO) were engineered for co-encapsulation of lipophilic bioactive compounds. High-pressure homogenization (HPH) was employed to modulate CSO size distribution and interfacial properties (surface hydrophobicity and protein conformation) of CSO. Results demonstrated that HPH treatment at 90 MPa dramatically reduced CSO-90 diameter from 1498.00 nm to 280.87 nm. This processing concurrently induced CSO interface protein unfolding, leading to significant increase in surface hydrophobicity and remarkable enhancement in curcumin (Cur) loading capacity. This size-tunable HPH processed CSO particles was complexed with chitosan (CS) and further used to stabilize high internal phase emulsions (HIPEs). The smaller CSO-90-CS particles was particular effective in stabilizing HIPEs co-encapsulating Cur and algal oil. This was probably facilitated by robust viscoelastic interfacial film formation. The resultant HIPEs exhibited enhanced functional properties, including increased gel strength, free radical scavenging capacity and oxidative stability (peroxide value reduced to 55 % of control; TBARS concentration maintained below 1.80 μg MDA/g algal oil during 8 days storage at 50 °C). This interfacial engineering strategy provides a promising platform for co-delivery and stabilization of oxidation-sensitive bioactive compounds, contributing to development of clean-label and nutrient-dense functional food products with enhanced physicochemical and bioactive stability.